By Diponkar Banerjee (auth.), Diponkar Banerjee, Sohrab P. Shah (eds.)
The earliest descriptions of human chromosomes initiated the genomics revolution that's now upon us. Array Comparative Genomic Hybridization: Protocols and Applicationsexplores the scope of what's now attainable so far as documenting abnormalities linked to a number of human cancers. whereas the expertise for interrogating the human genome keeps to conform, refinement of comparative genomic hybridization (CGH) utilizing array CGH and similar applied sciences have supplied huge, immense perception into human cancers at a cheap scale. Written within the profitable Methods in Molecular Biology™ sequence layout, chapters comprise introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, with ease reproducible protocols, and notes on troubleshooting and fending off identified pitfalls.
Authoritative and simply available, Array Comparative Genomic Hybridization: Protocols and Applicationsprovides researchers with well-honed methodologies to profit those thoughts for his or her personal use in examine or medical diagnostic laboratories.
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Additional info for Array Comparative Genomic Hybridization: Protocols and Applications
2. Methods for Array CGH Testing of Clinical Cancer Samples High throughput techniques such as ultradense aCGH are not only susceptible to the “garbage in-garbage out” principle but the vast amount of data produced in a semi-automated fashion amplifies the potential complications of producing poor quality data resulting in flawed interpretation. This is especially true when dealing with clinical samples which are often very small, sometimes partially degraded, and generally of heterogeneous composition.
And place it on another sterile petri dish. 4. Add 100 μl of extraction buffer to the biopsy. 5. Mince the tissue using a surgical scalpel blade. 6. Add 100 μl of extraction buffer and continue mincing the tissue (see Note 7). 7. Transfer the buffer and debris to a sterile Eppendorf tube and put it on ice. 8. Wash petri dish with another 100 μl of extraction buffer and add it to the Eppendorf tube. 9. Pass the suspension in the Eppendorf tube through a 20G needle five times using a syringe to disaggregate nuclei.
Gene Ruler 1 kb Ladder (Fermentas #SM0311). 3. For Biospecimen Collection and Processing Portable liquid nitrogen canister filled with liquid nitrogen. Powder-free gloves (latex). Bar-coded cryovials. Sterile tweezers. Cryogenic labels and permanent marker. Log sheets. Dry ice. T) compound, isopentane (2-methyl butane), cryostat, blades, glass slides, hematoxylin and eosin (H&E), etc. 4. If Sorting of Nuclei is Performed Sterile 35 × 10 mm petri dishes and sterile tweezers. 1%. Sterile single use stainless surgical blades (Lance Paragon LTD, Sheffield, England).